“... once the flames begin to catch the wind will blow it higher…”

Department of Chemistry and Biochemistry, College of Charleston, Charleston SC

My main interest is to understand how enzymes catalyze chemical reactions.

In my lab, we use kinetic measurements, chemical substitutions on enzymes and substrates, site-directed mutagenesis, heavy-atom isotopes, model systems, and structure-probing techniques to proceed towards this goal.

Students are exposed to several laboratory techniques, including PCR amplification, expression of proteins in E.coli cells, gel electrophoresis, radiolabeling of nucleic acids, HLPC, FPLC, and LC-MS.

Individual projects are described in the dedicated sections above; click here for a list of equipment.

Hammerhead Ribozyme PaSSU Intron SdsA1 Model compounds